Lab Methods, Reagents & Operations

Capping Protein (CapZ) Reagents

Monoclonal Antibodies at the Developmental Studies Hybridoma Bank

	Antibody name and link	Subunit / isoform recognized	Species recognized
	5B12	Alpha1 and alpha2	Chicken, mouse
	1E5	Beta1, not beta2	Chicken, not mouse or human
	3F2	Beta2, not beta1	Chicken, mouse, human

Expression Plasmids at

• Expression in Bacteria: Mouse, Chicken. Different isoforms. Wt and mutants.
• GFP Fusion Expression in Vertebrate Cells: Mouse Beta2

GFP-Actin Reagent

Light Microscopy Methods

• Affinity Purification of Antibodies
• Antibody Staining of Yeast
• Antibody Staining of Vertebrate Cells on Coverslips
• Phalloidin Staining of Yeast 
• Phalloidin Staining of Vertebrate
• Quantitation of Total Fluorescence per Cell - Background Correction with ImageJ
  • Instructions (pdf) and Spreadsheet (xls)

Protein Biochemistry Methods

• Actin Purification from Nonmuscle Sources
• Actin Purification - G150 Column
• Actin Prep from Muscle
• CapZ Prep from Muscle
• CapZ Prep from Bacterial Expression (pdf)
• Falling Ball Viscometry
• Pyrene Actin Prep

Lab Safety

• NFPA Fire Diamond