Differentially phosphorylated forms of the cortactin homolog HS1 mediate distinct functions in natural killer cells. Butler et al. 2008. Nature Immun 9: 887 - 897. Online Journal.

News and Views Article - Beemiller & Krummel. 2008. Distinct functions for HS1 in chemosensory versus adhesive signaling. Nature Immunology 9, 833 - 834.

Supplementary Text and Figures. Download PDF file (448KB)

Movie 1. Primary NK cells expressing GFP-HS1 in contact with K562 target cells. Frame interval 1 min. Download Movie (316KB)

Movie 2. Primary NK cells expressing GFP-HS1 in contact with K562 target cells. Frame interval 1 min. Download Movie (3MB)

Movie 3. Adhesion of primary control NK cells on ICAM-1. Brightfield, frame interval 5 sec. Download Movie (612KB)

Movie 4. Adhesion of primary control NK cells on Fn. Brightfield, frame interval 5 sec. Download Movie (2MB)

Movie 5. GFP-Actin dynamics in control NKL cells on ICAM-1. TIRF frame interval 3 sec. Download Movie (3MB)

Movie 6. GFP-HS1 dynamics in control NKL cells on ICAM-1. TIRF, frame interval 3 sec. Download Movie (5MB)

Movie 7. Adhesion of HS1 knockdown primary NK cells on ICAM-1Adhesion of primary NK cells on Fn. Brightfield, frame interval 5 sec. Download Movie (1MB)

Movie 8. Adhesion of HS1 knockdown primary NK cells on Fn. Brightfield, frame interval 5 sec. Download Movie (2MB)

Movie 9. GFP-Actin dynamics in HS1 knockdown NKL cells on ICAM-1. TIRF, frame interval 3 sec. Download Movie (576KB)

Movie 10. Adhesion of HS1 knockdown primary NK cells expressing Y378F HS1 on ICAM-1. Brightfield, frame interval 5 sec. Download Movie (2MB)

Movie 11. GFP-Actin dynamics in HS1 knockdown NKL cells expressing Y378F HS1 on ICAM-1. Brightfield, frame interval 5 sec. Download Movie (7MB)

Movie 12. Adhesion of HS1 knockdown primary NK cells expressing Y397F HS1 on ICAM-1. Brightfield, frame interval 5 sec. Download Movie (628KB)

Movie 13. GFP-Actin dynamics in HS1 knockdown NKL cells expressing Y397F HS1 on ICAM-1. TIRF, frame interval 3 sec. Download Movie (212KB)

Movie 14. Chemotaxis of control primary NK cells on Fn. Brightfield, frame interval 1 min. Download Movie (3MB)

Movie 15. Chemotaxis of HS1 knockdown primary NK cells on Fn. Brightfield, frame interval 1 min. Download Movie (6MB)

Movie 16. Chemotaxis of HS1 knockdown primary NK cells expressing Y397F HS1on Fn. Brightfield, frame interval 1 min. Download Movie (18MB)

Movie 17. Chemotaxis of HS1 knockdown primary NK cells expressing Y378F HS1 on Fn. Brightfield, frame interval 1 min. Download Movie (25MB)

Movie 18. GFP-Actin dynamics during chemotaxis in control NKL cells on Fn. TIRF, frame interval 15 sec. Download Movie (872KB)

Movie 19. GFP-Actin dynamics during chemotaxis in HS1 knockdown NKL cells on Fn. TIRF, frame interval 15 sec. Download Movie (1MB)

Movie 20. GFP-Actin dynamics during chemotaxis in HS1 knockdown NKL cells expressing Y397F HS1 on Fn. TIRF, frame interval 15 sec. Download Movie (7MB)

Movie 21. GFP-Actin dynamics during chemotaxis in HS1 knockdown NKL cells expressing Y378F HS1 on Fn. TIRF, frame interval 15 sec. Download Movie (2MB)